This quantity offers correct single-cell and single-molecule ways within the learn of microbes generating and using hydrocarbons and lipids. whereas generically acceptable for all microorganisms, the methods defined are, anyplace attainable, tailored to the sphere of research of hydrocarbon and lipid microbiology. The equipment comprise uncomplicated methods for keeping apart unmarried cells by way of microfluidics and stream cytometry, and their cultivation in arrays as natural clones; for keeping apart, amplifying and sequencing single-cell genomes and transcriptomes; and for analysing single-cell metabolomes through Raman spectroscopy. Single-molecule ways contain using protein:fluorescent dye fusions for protein localization and strategies for the creation of mobilephone department protostructures and lipid monolayers. tools for the practical research of unmarried cells contain detection of metabolically energetic (protein-synthesizing) cells in environmental samples by means of bioorthogonal non-canonical amino acid tagging, Raman spectroscopy mixed with sturdy isotope labelling and fluorescent in situ hybridisation, and visualization of unmarried cells partaking in gene move job. finally, protocols are awarded for single-cell biotechnological purposes, together with biofuel production.
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